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The therapeutic effects of AB4 on SALI were evaluated through histopathological examination, biochemical analysis, immunofluorescence staining, and Western blotting, which collectively elucidated its in vivo and in vitro mechanisms of action

Despite the limited research available on the interplay between mitochondria and iron overload, their critical function in maintaining iron homeostasis and managing associated damage underscores their importance in the pathological processes of both congenital and acquired iron overload

Joyal JS, Sun Y, Gantner ML, Shao Z, Evans LP, Saba N, et al

doi: 10.1038/s41423-024-01220-3 117 CaoQWangYChenJWangRChenTGlossBet al

The complex was concentrated to 0.5 ml using Ultra Centrifugal Filter (ThermoFisher MWCO, 100 kDa) and subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20 mM HEPES pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 5 M ligands, 0.00075%(w/v) LMNG, 000025% glyco-diosgenin (GDN, Anatrace) and 0.00015% (w/v) CHS to separate complex from contaminants