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After the mixture was washed 5 times with a buffer composed of 50 mM HEPES-KOH (pH 7.5), 100 mM NaCl, 1% Triton X-100, 0.5% deoxycholate, 0.1% SDS, 1 mM EDTA, and a protease inhibitor cocktail (EDTA-free), bound proteins were eluted with 0.1 M citrate and subjected to Western blotting with an anti-Kras antibody (1:250 dilution

observed that compared with the sham group, mice undergoing the AKI-to-CKD transition displayed decreased abundance of Actinobacteria and Firmicutes [169]

Treatment focuses on supporting the body's natural detoxification pathways through a combination of targeted nutritional supplements, binders such as charcoal and clay to trap toxins in the gut, and lifestyle modifications

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