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This process not only promotes STAT3 phosphorylation and nuclear translocation but also aggravates intestinal inflammatory response and intestinal barrier damage, Therefore, controlling the intake of LCFAs is a potential strategy for the prevention and treatment of IBD (144)

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For amino acid refeeding, cells were restimulated for 30 min with a 1 water-solubilized mix of essential (11130036, Thermo Fisher Scientific) and non-essential (11140035, Thermo Fisher Scientific) amino acids resuspended in amino-acid-free DMEM supplemented with 10% dialysed FBS plus glutamine

You might feel some burning or stinging during the injections, but it fades quickly