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We examined if NAC may prevent the pathophysiological impacts of THC using a rodent model of adolescent brain development and chronic THC exposure

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Moreover, the RNA samples were subjected to reverse transcription polymerase chain reaction (RT-PCR) in order to verify that they were suitable for complementary DNA (cDNA) synthesis and not significantly contaminated by guanidine salts or ethanol, which interfere with the downstream microarray hybridization

Because the shortened peptide product is also subject to enzymatic cleavage, care must be taken to control the conditions of reaction so that the products of successive cleavages are properly monitored