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Transient overexpression of hs GNMT and associated mutants was achieved with calcium phosphate transfection of the corresponding pcDNA3.3 mammalian overexpression plasmid, which was modified to include an internal ribosome entry site (IRES) downstream of the open reading frame to allow for bicistronic expression of GFP as a fluorescent marker of overexpression

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Although an increasing number of metabolomics studies, an emerging analysis technique, have linked AAD to different low-molecular-weight-metabolites (LMWMs) [9, 10], there is still a lack of a comprehensive understanding of the metabolic profile of AAD has hampered our understanding of the biology of the hallmark metabolites and their role in AAD pathogenesis

doi: 10.3390/jcm8020232 17 ChaudhariV