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Selenium is an important dietary constituent of at least 25 human selenoproteins and enzymes containing selenocysteine [41,42,43]

Typically, cells were seeded in cell-culture dishes at a density of 1 10 5 cells/dish, and cultured overnight

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Finally, antimycin A and rotenone were applied to block the electron transport chain, thereby quantifying the non-mitochondrial respiration and assessing the residual respiration due to other cellular activities