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Genus-level networks (Figure S6B) identified high-centrality nodes such as Paludicola and Staphylococcus, suggesting their roles as keystone taxa

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Lysate from His-tagged proteins were mixed with Ni-NTA beads (Qiagen) 2 h, and washed with lysis buffer supplemented with 25 mM imidazole

In the study by iauskait and his team, highly metastatic human colon carcinoma cells were treated with sulforaphane, a bioactive compound found in cruciferous vegetables, in combination with the FOLFOX regimen (a combination of 5-FU, leucovorin, and oxaliplatin) to demonstrate an additive anticancer effect