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Lavar con 3 mL de solucin acuosa de cido clorhdrico 10 mmol/L, descartar el lavado, secar al vaco durante 1 min, eluir con 2 mL de solucin de metanol con amonaco al 4%, y recoger el eluato en un tubo de centrfuga de 15 mL

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cosolvents include for example, alcohols, ethers, glycol ethers, alkanes, alkenes, linear and cyclic amides, perfluorinated tertiary amines, perfluoroethers, cycloalkanes, esters, ketones, aromatics, the fully or partly halogenated derivatives thereof and mixtures thereof

doi: 10.1016/j.trecan.2021.02.004 Summary Keywords metabolic reprogramming, phosphoglycerate dehydrogenase (PHGDH), serine metabolism, serine synthesis pathway (SSP), targeted therapy, tumor immune microenvironment Citation Su P, Yang Y and Zheng H (2026) The serine code of metabolic reprogramming: multidimensional roles of the serine synthesis pathway in tumors and novel breakthroughs for targeted therapy