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Densitometry was evaluated using ImageJ software

Cell Death Dis 14(4):281 Lee S et al (2023) Autophagy mediates an amplification loop during ferroptosis

The membranes were sequentially probed with the primary antibodies (1:5000) at 4 C overnight and corresponding horseradish peroxidase-conjugated secondary antibodies (1:10,000, Pierce at room temperature for 1 h)

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