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RSL3 contains a reactive chloroacetamide moiety that makes it a potent and irreversible GPX4 inhibitor, although its poor solubility and poor absorption, distribution, metabolism, and excretion (ADME) properties limit its effectiveness in in-vivo studies [22]

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Nature 399:362366 Hu Q, DAmora DR, MacNeil LT, Walhout AJM, Kubiseski TJ (2018) The Caenorhabditis elegans oxidative stress response requires the NHR-49 transcription factor