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Most of the studies conducted on IBP replication have used as host cells different established cell lines, including MO- and MP-like cell lines (e.g., J774A.1, P388.D1, RAW264.7, THP-1, U-937) as well as epithelial and fibroblast cell lines (e.g., Caco-2, HeLa, Hep-2, HEK293, MDCK, NIH3T3, and others)

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Thus, the pH of the solvent can be adjusted to facilitate binding to IEX or to promote elution of a bound protein

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Watts GF, Castelluccio C, Rice-Evans C, Taub NA, Baum H, Quinn PJ