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Primary cortical neurons were maintained in Neurobasal medium (Life Technologies Corporation) supplemented with 1x B-27 (life technology)

The reaction was incubated 1 h at room temperature, diluted with 20 mM Tris pH 8.0, diafiltrated, rediluted and the protein digested by trypsinization for 3 h

National Institutes of Health, Bethesda, USA) by measuring the mean pixel intensity within the selected region of interest in 16-bit images and is expressed in arbitrary units (AUs) in Fig

To explore the molecular basis for these potential changes, we cross-referenced 1178 upregulated DEGs from Dmp1-Cre

Even more impressively, intracellular glutathione inside peripheral blood mononuclear cells (PBMCsa critical type of immune cell) increased by an astounding 100%