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Pharmacol Biochem Behav 50 : 147151 Gambarana C, Masi F, Tagliamonte A, Scheggi S, Ghiglieri O, De Montis MG

To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit