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Cells were grown at 37 C to an optical density at 600 nm (OD 600 ) of 0.70.8 in lysogeny broth (LPS solution LB-05) medium, and then 0.5 mM of isopropyl-1-thio--D-galactopyranoside (LPS solution IPTG025) was added

It was identified in the late 1980s and initially attracted attention because its deposits are found in the pancreases of people with type 2 diabetes

Subsequently, the proportion of tritium water increased and that of [ 3 H]proline decreased gradually with time

furthermore, the in vitro migration and rate of spreading of tendon fibroblasts increased in a dose-dependent manner, which was attributed to the activation of the FAK-paxillin pathway