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The de novo synthesis of GSH takes place in the cytosol of human cells and involves two sequential ATP-dependent enzymatic reactions 9

An interaction with glutathione/glutaredoxin has been observed in all roGFP-based sensors generated to date [19, 31, 4549] and it is unclear why this interaction should not also occur in the context of the NERNST probe

These bioactive components are believed to ameliorate diabetic syndromes through six main mechanisms of action, including enhanced insulin secretion and sensitivity, muscle cell and adipose tissue glucose uptake, inhibition of intestinal glucose absorption and hepatocyte glucose production, and anti-inflammatory qualities [7]

Statements Author contributions FM: Conceptualization, Investigation, Writing original draft, Writing review and editing

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