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The answer may lie in differences in affinities of folate transporters and receptors for folic acid compared with reduced folates

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While most patients tolerate B12 shots well, mild side effects can occasionally occur, including minor pain or redness at the injection site, mild diarrhoea, or, in rare instances, an allergic reaction

This complex interplay provides a mechanistic explanation for the productivity loss observed in long-term cultures, importantly highlighting rational metabolic levers for media reformulation, such as trade-offs in cysteine utilization and alterations in one-carbon (C1) metabolism

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