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Description

The cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin G, and 100 g/ml streptomycin at 37C in a humidified 5% CO2-95% air incubator under standard conditions

KacniovMVukoviNChleboRHakPRovnaKCubonJet al

FAD measurements A total of 1 10 6 FSP1 GFP-P2A-BFP or FSP1 KO cells expressing the indicated FSP1GFP construct and/or sgRNA were seeded into 10-cm 2 plates