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Their core mechanism involves regulating the composition of gut microbiota to increase concentrations of SCFAs and secondary BAs, thereby activating ECCs to release 5-HT, enhancing ACh secretion from cholinergic neurons, and optimizing ENS inhibitory neural signals via the neuronal nNOS pathway, ultimately promoting gastric emptying and regulating intestinal transit rhythms (Huang et al., 2023

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The finding was further supported by c-met-binding experiments, in which HGF was pre-treated with GSSSG and LASSS at the same molar ratio 1:4000 at which the satellite-cell activation index was rescued to a level comparable to that of non-nitrated regular HGF, as shown in Figure 1d, followed by exposure to peroxynitrite (1:2000) and evaluated for c-met-binding by a sandwich ELISA-like assay on recombinant c-met-Fc chimera (Figure 1e, left column

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For chronic conditions or maintenance, 8-12 week protocols are common