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Adv Ther 42 , 12511264 (2025)

Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)

In SURPASS-2, the rates of all reported GI side effectsincluding nausea, diarrhoea, vomiting, dyspepsia, decreased appetite, constipation and abdominal painoccurring with tirzepatide (4044.9%) were generally comparable to those seen with semaglutide (41.2%) [9]

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Symptoms include bone pain, muscle weakness, frequent colds, low energy, and mood disorders like depression and anxiety