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The main advantage of omitting trypsin use from MS sample preparation is that peptide ends are likely to arise from endogenous protease cleavage sites, thus increasing sensitivity for identification of relevant peptide proteoforms 19

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For each sample, 120 L of plasma was aliquoted into two round-bottom 96-well plates, kept on dry ice, sealed with plastic film, and stored at 80 C until LC-MS/MS sample preparation and analysis

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