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A portion of brain tissue (100 mg) was taken in a 900-microliter volume of PBS (1:9 ratio) in a glass homogenizer on ice and homogenized at 2,000 rpm for 5 min

HEK293T cells were grown to 70% confluency in 96-well plates (Corning) at 37C in DMEM supplemented with 10% FBS, and treated with Entranster TM -H (Engreen) transfection reagent (0.1 L) and purified plasmids [0.25 g pGL4.74 (hRluc/TK) and 0.25 g pGL4.32(luc2P/NF-B-RE/Hygro plasmid)] in 50 L DMEM/10% FBS per well

doi: 10.1136/jcp.2007.050054 47 NashPChardMHazlemanB

moellendorffii , we conclude that these genes have rapidly and dramatically expanded between bryophytes and lycophytes

From root to fruit: rNA-Seq analysis shows that arbuscular mycorrhizal symbiosis may affect tomato fruit metabolism