PNAS 103:56085613 Delaunay A, Pflieger D, Barrault MB, Vinh J, Toledano MB (2002) A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation
Clinical Journal of Sports Medicine 22(2) :172-208
For example, mitochondrial dysfunction destabilizes lysosomes, amplifying oxidative stress ( 2.2.2 Endoplasmic reticulum: regulator of lipid metabolism and redox homeostasis The ER maintains cellular homeostasis through protein synthesis, lipid metabolism, and calcium storage ( - localized to the ER membranemediates cystine import for GSH synthesis ( 2.2.3 Lysosomes: controllers of iron homeostasis Lysosomes regulate cellular debris degradation, biomolecule recycling, and iron metabolismall of which influence ferroptosis ( 2.2.4 Lipid droplets: sources of polyunsaturated fatty acids Lipid droplets (LDs) are dynamic organelles serving as storage reservoirs for neutral lipids, playing pivotal roles in cellular energy homeostasis and lipid metabolism ( 2.2.5 Peroxisomes: modulators of lipid oxidation and antioxidant defense Peroxisomes regulate lipid metabolism and ROS detoxification, influencing ferroptosis ( 2.2.6 Golgi apparatus: regulatory mechanisms in ferroptosis The Golgi apparatus is a vital organelle involved in the post-translational modification, sorting, and trafficking of proteins and lipids within the cell ( Collectively, the dysfunction of key organellesincluding mitochondria, endoplasmic reticulum, lysosomes, lipid droplets, peroxisomes, and the Golgi apparatussynergistically amplifies ferroptosis susceptibility in COPD
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After washing (3) in 0.01 M PBS (pH 7.2), the slides were incubated with secondary antibody (horse anti-mouse/rabbit IgG) for 30 min at 37C
