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Marie were collected both upstream and downstream of Michigan and Ontario sewage treatment plants

Subsequently, slides were washed 3X with PBS (10 min, RT) and incubated with the secondary horseradish peroxidase conjugated antibody (mouse anti-rabbit IgG, 0.4 mg/mL diluted 1:500, sc-2357 or goat anti-mouse IgG, 0.4 mg/mL diluted 1:500, sc-2031) for 2 h at RT, followed by three PBS washes and treated with the 3,3-diaminobenzidine substrate (ab64238, Abcam) for 10 min

This condition may be treatable before miscarriage ensues if diagnosed early on and not caused as a result of genetics or deformity

Although rare, patients with a history of skin sensitivity or allergies should be monitored closely for any adverse reactions

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