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The thermocycler conditions were as follows: 95C for 10 min, followed by 40 cycles of 95C for 15 sec, 65C for 15 sec and 72C for 45 sec, ending with 1 cycle of 72C for 10 min

These evaluations allow for timely adjustments to dosing, supportive therapies, and recovery strategies while tracking progress throughout the treatment program

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