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Nair, and Shyam Diwakar, Cerebellum in Neurological Disorders: A Review on the Role of Inter-Connected Neural Circuits, Journal of Neurology and Stroke, vol

doi: 10.1371/journal.pone.0153427 14 WeaverLHamoudARStecDE

Quality filtered reads were mapped on assembled contigs to obtain abundance profiles, which were normalized to CPM (Counts Per Million) using EdgeR v3.10.2 35

The protein was concentrated to ~ 12 mg ml 1 in 25 mM tris (pH 8.0), 250 mM NaCl, and 2 mM tris(2-carboxyethyl) phosphate (TCEP) and stored in aliquots at 80 C

This increased period of receptor agonization is responsible for a hyperexcitation of the cholinergic nervous system leading to individual changes and finally death