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The beads were washed four times with high-salt wash buffer (10 mM HEPES, pH 7.9, 25% glycerol, 1.5 mM MgCl 2 , 300 mM KCl, and 0.1% Triton X-100), followed by two washes with low-salt wash buffer (10 mM HEPES, pH 7.9, 25% glycerol, 1.5 mM MgCl 2 , 100 mM KCl, and 0.1% Triton X-100)

AMPK inhibition augments energy deficiency-induced eryptosis [149]

The ethylene-responsive transcription factor of durum wheat, TdSHN1, confers cadmium, copper, and zinc tolerance to yeast and transgenic tobacco plants

4F ), which further proved that HSP90 plays a vital role in the process of Tim-AIII-induced ferroptosis

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