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However, you can use 2 mL (5 mg/mL), 4 mL (2.5 mg/mL), or 5 mL (2 mg/mL) depending on your needs

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500 l of cells were incubated for 30 min at 28 C and 650 rpm in a ThermoMixer, in the presence of 200 nM MitoTracker Green FM (Molecular Probes, Leiden, The Netherlands), washed with fresh medium and imaged at the microscope

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demonstrated that GSH plays an important role in hamster oocyte spindle function