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hPSCs were dissociated into single-cell suspensions using TrypLE Select (Thermo Fisher Scientific, Netherlands) and plated at a density of 2 10 6 cells/well in Stem Flex medium (Thermo Fisher Scientific, Netherlands) supplemented with 10 M Y-27632 (Peprotech, USA) on Geltrex-coated 6-well plates (Eppendorf, Germany) under a 5% CO 2 atmosphere

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