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Several factors influence the choice of needle and syringe: Type of injection : Intramuscular (IM) or subcutaneous (SC) Gauge of the needle : Diameter of the needle Length of the needle : Determines the depth of injection Volume of the syringe : Determines the amount of B12 to be injected 3

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After 24 h, the medium was replaced with RPMI medium, and cells were monitored for wound closure over 48 h

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