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Enzyme-linked immunosorbent assay To detect binding of complement components or plasminogen, microtiter plates (Nunc MaxiSorp, Thermo Fisher Scientific) were coated with 100 l of purified His 6 -tagged proteins (5 g/ml) or BSA (5 g/ml) in PBS at 4 C overnight 74

For critically ill patients, intestinal-targeted therapy necessitates a delicate balance between efficacy and safety

Agilent, Walnut Creek, California, USA) using an 8-channel transmit/32-channel receive head coil at the Center for Functional and Metabolic Mapping of Western University in London, Ontario

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10.1128/JVI.02380-05 203