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Total proteins from lysed cells or patient tissue were extracted using RIPA lysis buffer and electrophoresed by SDS-PAGE and transferred onto PVDF membranes subsequently

Since Diclofenac metabolites are mainly eliminated through the kidney, the patients with renal failure should be controlled closer than those with normal renal function

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52,53 Furthermore, the activation of inflammasomes mainly manifests as an elevation of serum IL-1 and interleukin-18 (IL-18) and the recruitment of inflammatory cells