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low MYCN ( + Dox) ( n = 3 samples, experiment replicated 3 times)

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Further purification was performed by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (Cytiva) equilibrated with a buffer containing 25 mM MES-NaOH pH 6.5, 150 mM NaCl, 0.01% (w/v) LMNG, and 0.001% (w/v) CHS

Preserving muscle mass through resistance training and adequate protein intake is critical, as rapid weight loss can reduce testosterone-supporting lean tissue