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Furthermore, treatment of cells with 22(R)-diol and pregnenolone and ensuing oxidative stress resulted in decreased mRNA levels of StAR and CYP11A1 and increased mRNA levels of antioxidant enzymes SOD1, SOD2 and CAT

mRNA of Pparg, Ppara, Cpt1a and fatty acid oxidation The relative abundance of mRNA transcripts encoding for Pparg , Ppara and Cpt1a were examined by quantitative RT-PCR