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After incubation, the suspension was centrifuged at 2000 rpm for 5 min, and the supernatant was measured at 540 nm using a microplate reader

10.1016/j.fct.2023.113723 87 LuC.XuW.ShaoJ.ZhangF.ChenA.ZhengS

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The secondary mass resolution was set at 15000@m/z 200, with an AGC target of 1e6, a secondary maximum IT of 25 ms, HCD as the MS2 activation type, an isolation window of 20 Th, and a normalized collision energy of 32 98,99

Therapeutic potential of SIRT1 and NAMPT-mediated NAD biosynthesis in type 2 diabetes