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Because AOD9604 is studied in the context of selective fat metabolism modulation, adipocyte function regulation, and body composition effects, it is a subject in experiments related to metabolic health and weight regulationwithout implying outcomes outside of a research setting.

3T3-L1 cells were cultured in 60 mm diameter dishes (8.4 104 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)