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Whole blood or plasma: what is the ideal matrix for pharmacokinetic-driven drug candidate selection

134:113 Jasinski et al (1985): Clonidine in morphine withdrawal

Neuroradiology 2001;43:139-143

Effect of increasing the ionic strength and diluting the cell lysate in the presence and absence of BugBuster without sonication Induced cells were next used in triplicates and re-suspended independently in LSB-2 containing 75, 150 or 300 mM NaCl keeping all the other components (10 mM Tris, 1 mM EDTA, 10 mM -mercaptoethanol and 1 mM PMSF) intact, followed by incubation with BugBuster and processed without any sonication

[45:09] Jamie But you are