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At least five animals were then individually picked into a new Eppendorf tube with M9 buffer and homogenized using a motorized tissue grinder (Fisher Scientific) coupled with a 1.5 mL plastic pestle (Fisher Scientific)

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Bone and musculoskeletal research: Some work has extended into bone repair and osteoporosis models, where AOD9604 has shown early signs of supporting bone remodeling, though this area is less developed than its fat metabolism and cartilage research