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Ethics statement The protocol of animal experiments was approved by the Ethics Committee of Zhejiang Baiyue Biotech Co., Ltd., for Experimental Animals Welfare (approval number: ZJBYLA-IACUC-20220901) and carried out under the guidelines of the National Institutes of Health on Animal Care and Use

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(2025) demonstrated that inhibiting EW/CART neurons reduces alcohol intake, further underscoring their role in alcohol consumption regulation 84 .The alcohol-induced response in EWcp was postulated to be a result of signaling via GABA-A receptors, modified by 2A/D-adrenoceptors and dopamine receptors 20 , we propose that TRPA1 may also contribute 41

Affinity chromatography which provides analysis of bioactive molecules based on their biological functions or individual structures has become increasingly important as another liquid chromatography technique [1,12]

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