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One microgram of total RNA from each sample was reverse transcribed, and the real-time RT-PCR measurements were performed, as described previously [49], using an apparatus Aria MX (Agilent Technologies, Santa Clara, CA, USA) with the corresponding SYBR Green kit, according to the Promega manufacturers recommendations

It is constitutively expressed at a high level in different mammalian cell lines and normal tissues, but the specific transcription factors involved in the regulation of its expression have not yet been completely identified

Glutamine metabolism in the proliferation of GS-expression pituitary tumor cells

Tape stripping has been utilised as a pre-treatment to enhance medication penetration and as an analytical technique to evaluate the depth and uniformity of drug permeation into the skin

Biochemical tests for AGHD diagnosis are imperfect, and their accuracy is strongly affected by the pre-test probability of the condition