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The cells were harvested by centrifugation (1000 x g , 2 min) and resuspended in lysis buffer (5% w/v 5-sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA (KPE buffer [35])) together with 425600 m-diameter acid-washed glass beads (Sigma) at a buffer/bead ratio of 1:2 by volume

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doi: 10.1056/NEJMoa2116846 105 GouldR

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Conversely, many everyday medications are potent inhibitors of these same enzymes, creating the potential for complex, bidirectional interactions