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Understanding the Causes of Black Patches on Skin

10.1371/journal.pone.0109442 58 DinglasanR

Measurement of GSSG with or without GSH masking reagent Interference Reducing agents such as ascorbic acid, -mercaptoethanol, dithiothreitol (DTT) and cysteine, or thiol reactive compounds such as maleimide compounds, interfere with the glutathione assay

This was proposed to be driven through increased Bax and concurrently decreased B-cell lymphoma/leukemia type 2 protein (Bcl-2) levels following inhibition of NFkB in addition to TMZ treatment [105]