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These moderated t-tests in DESeq2 are shrinkage-based two-sided Wald tests (H 0 : log 2 FC = 0), which incorporate empirical Bayes moderation to improve dispersion estimation across genes, allowing for more reliable inferences 85

10.1016/j.it.2019.02.001 [DOI] [PubMed] [Google Scholar] Nuhu F., Gordon A., Sturmey R., Seymour A

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