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doi: 10.1038/nrmicro.2015.25 71 KazemianN.KaoD.PakpourS

Similarly, Gly-Leu-Pro, an ACE inhibitory peptide from chum salmon skin gelatin, exhibited an antihypertensive effect in spontaneously hypertensive rats, reducing systolic blood pressure by 31 mmHg after 6 h at a dosage of 20 mg/kg [141], suggesting that it could serve as a promising natural alternative for the development of functional foods aimed at controlling hypertension

The samples were incubated for at least 10 min at 25C before the addition of 50 l of 0.5% N -ethylmaleimide

The cell pellets were then washed twice with 1X phosphate buffer (0.1 M, pH 7.4)