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Finally, the beads were washed 5 times with 1 ml of modified binding buffer (20 mM HEPES-KOH (pH = 7.9), 200 mM KCl, 0.2 mM EDTA, 1 mM DTT, 15 % glycerol, 0.2 % NP-40) and stored in 10 mM Tris-HCl (pH=8.1) at 80 C

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In summary, L-carnitine improves MASLD through multiple mechanisms including mitochondrial protection, oxidative stress reduction, and autophagy/apoptosis suppression

Mutation analysis identified four glutamine residues in the GST tag as polyamination sites

Glutathione is unique among antioxidants because it directly scavenges nitric oxide (NO), a neurotransmitter critical for coordinating peristaltic waves in the gut