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The bound endogenous GS was eluted from cross-linked IgG using Elution buffer pH 2.8 as per the manufacturers protocol

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Exposing cerebral mitochondria to THC was reported to induce a ten-fold increase in free radical leak and increased H 2 O 2 production [40], while in epithelial cells, THC upregulated inflammation-related genes and downregulated antioxidant-related genes [34]