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Dowdle et al

RNA isolation, reverse transcription, and quantitative real-time PCR (qPCR) Total RNA was extracted using Trizol (Invitrogen, Waltham, MA, USA)

Discovery of MD-224 as a First-in-Class, Highly Potent, and Efficacious Proteolysis Targeting Chimera Murine Double Minute 2 Degrader Capable of Achieving Complete and Durable Tumor Regression

APLB Glutathione Niacinamide Sheet Mask (Set of 10) helps brighten, hydrate, and refresh dry, dull, and tired skin

2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C