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EGF promotes skin regeneration, resulting in smoother and more youthful skin

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J Clin Endocrinol Metab 83:16541661 Ding H, Gao XL, Hirschberg R, Vadgama JV, Kopple JD (1996) Impaired actions of insulin-like growth factor 1 on protein synthesis and degradation in skeletal muscle of rats with chronic renal failure

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Omission of the reducing step, which would prevent the release of glutathione residues and the generation of free sulfhydryl groups, resulted in absence of fluorescence ( Figure 2B ), supporting efficiency of the blocking step