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Sometimes DNase I and/or EDTA are included in the final cell suspension medium to reduce any cell aggregation

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While it has been previously suggested that the ancestor of the Onygenales very likely was heterothallic 39 , the placement of multiple lineages of homothallic species in this group raises the possibility of a homothallic ancestor, with more recent transitions in pathogenic species to be heterothallic and rearrangements of the mating locus in others such as P