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High-quality oocytes, the prerequisite for successful pregnancy, are closely related to mitochondrial function and an enzymatic environment that balances intracellular ROS levels (106)

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NMDA activation also requires binding of co-agonist glycine or D-serine to a distinct site on the receptor, providing an additional level of regulation

Concentrations typically used in cell culture range from 10 ng/mL for sensitive signaling assays to 100 ng/mL for full receptor saturation studies