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In the current study lowest numbers of animals and surgeries were done in a clean situation ( 2.3 Chronic constriction injury To evaluate the effect of AST on neuropathic pain, the CCI was induced according to the Bennett method ( 2.4 Experimental groups In total, 54 rats were divided into nine groups of six, including, the sham group (undergoing surgery without CCI and receiving DMSO 5%), CCI group (undergoing CCI and receiving DMSO 5%), gabapentin (positive control group, 100 mg/kg in DMSO 5%), AST-treated groups (5 and 10 mg/kg in DMSO 5%), flumazenil (FLU) (0.5 mg/kg), naloxone (NAL) (0.1 mg/kg), AST (10 mg/kg) + flumazenil (0.5 mg/kg) (AST + FLU), and AST (10 mg/kg) + naloxone (0.1 mg/kg) (AST + NAL) (Supplementary Figures 1, 2)